Biology

Directions: Download and Save this document as “BIOL 1020 Unit 2 Module 5 Lab Assessment 1_Lastname”. SAVE YOUR WORK AS A WORD (.doc)DOCUMENT. When you have completed this assessment, submit it to the appropriate Assignments area in Blackboard.Using the VUMIE Lab Manual, the VUMIE website, your notes, and in some cases outside research, answer the following questions. Youranswers must be in the form of complete and grammatically correct sentences. Highlight your responses or use a different font color(todistinguish them from the questions themselves). These questions are ordered as you proceed through the VUMIE lab exercise sections.10 pointsIdentifying Gram Positive Cocci1) A good starting point would be to use the Help Files, particularly the “Show Me How To…” files related to identify bacteria.Work through the Help files and you will find a recommended approach to get started on the ID process for identifying your Grampositive coccus. What tests would you perform initially to start you on your way?2) A key “first test” used in a clinical setting is evaluating growth characteristics on 5% sheep blood to detect presence ofenzymes that destroy and digest blood cells and heme. Find the Help file information on blood hemolysis to learn more about this test.What are the three possible results for the test?3) Just for fun, click on Unknown and select Record Results. We are going to assign results without doing the tests. Find AlphaHemolysis or Beta Hemolysis and record a result for one of these as positive. Watch as the number of columns is reduced by the resultsyou enter. Why would growth characteristics on blood agar be an important start for identifying bacteria in a hospital lab?4) Repeat the manual input of test results for the bile esculin slant. Record both as positive. Note the change in the number ofcolumns as these results are entered. Why are positive results with bile esculin important information toward understanding the normalecology of the organism being tested?5) What would be the next test you would perform on the unidentified Gram positive coccus to help provide a quick identification?Directions: Download and Save this document as “BIOL 1020 Unit 2 Module 5 Lab Assessment 5_Lastname”. SAVE YOUR WORK AS A WORD (.doc)DOCUMENT. When you have completed this assessment, submit it to the appropriate Assignments area in Blackboard.Using the VUMIE Lab Manual, the VUMIE website, your notes, and in some cases outside research, answer the following questions. Youranswers must be in the form of complete and grammatically correct sentences. Highlight your responses or use a different font color(todistinguish them from the questions themselves). These questions are ordered as you proceed through the VUMIE lab exercise sections.10 pointsMannitol Salt Agar1) Remove the culture from the incubator and observe the growth. Provide results for these two tests (Circle or highlight):Growth on 7.5% NaCl: + –Mannitol Fermentation: + –2) What result would be expected in the “growth in 6.5% NaCl test” for bacteria capable of growth on mannitol salt agar? Explain.3) Find the recipe for mannitol salt agar. List each of the components in the space below. For each component, explain whether it isdifferential, selective, both differential and selective, or none (meaning it does not distinguish between any bacterial types).Directions: Download and Save this document as “BIOL 1020 Unit 2 Module 5 Lab Assessment 2_Lastname”. SAVE YOUR WORK AS A WORD (.doc)DOCUMENT. When you have completed this assessment, submit it to the appropriate Assignments area in Blackboard.Using the VUMIE Lab Manual, the VUMIE website, your notes, and in some cases outside research, answer the following questions. Youranswers must be in the form of complete and grammatically correct sentences. Highlight your responses or use a different font color(todistinguish them from the questions themselves). These questions are ordered as you proceed through the VUMIE lab exercise sections.10 pointsBlood Hemolysis1) Using the information provided above (and elsewhere if so instructed) record the type of hemolysis present in the software andhere (Circle or highlight):Alpha Beta Gamma2) Streak the plate and incubate it at 37°C overnight. Retrieve the plate and right click to “Record Results”. Are the halostransparent or a discoloration of the medium? (Circle or highlight):Transparent DiscolorationRecord your results in the software and here (Circle or highlight):Alpha Beta Gamma3) Use the Help files and Identification Matrix to provide answers to the following:a. Which form of hemolysis is most frequently associated with these microbes?Microbe Hemolysis typeStaphylococcus aureusStreptococcus pyogenesStreptococcus pneumoniaStreptococcus mutansEnterococcus faecalisStaphylococcus epidermidisb. How is blood agar related to chocolate agar?c. How might the presence of hemolysins enable a microbe to be more virulent than one without hemolysins?Directions: Download and Save this document as “BIOL 1020 Unit 2 Module 5 Lab Assessment 4_Lastname”. SAVE YOUR WORK AS A WORD (.doc)DOCUMENT. When you have completed this assessment, submit it to the appropriate Assignments area in Blackboard.Using the VUMIE Lab Manual, the VUMIE website, your notes, and in some cases outside research, answer the following questions. Youranswers must be in the form of complete and grammatically correct sentences. Highlight your responses or use a different font color(todistinguish them from the questions themselves). These questions are ordered as you proceed through the VUMIE lab exercise sections.10 pointsBile Esculin1) Remove the medium from the incubator and record the results in the software for the Virtual Lab Report. Also record the resultsin the table below. If you need help interpreting the test, click on the “M” icon and to read up on how the medium is used. You canlook up the tests for which it is used by clicking on the “T” icon and reading up on those tests.Biochemical Tests Staphylococcus aureusMedia Tests 5 StaphylococcussaprophyticusMedia Tests 6Growth on BileEsculin Hydrolysis2) Which of the following bacteria would be most likely to encounter bile salts in their normal habitat? (Circle or highlightallcorrect answers)Staphylococcus aureus Enterobacter aerogenes Enterococcus faecalisStreptococcus pyogenes Escherichia coli Staphylococcus epidermidisWhy would those you selected be expected to grow on bile esculinagar?3) Where in the digestive system are lipids digested? Is there any evidence that these bacteria are more inclined to causeinfections in those organs than are bacteria incapable of growing on bile?4) It is possible to have an organism that is positive for both “growth on bile” and “esculin hydrolysis”, an organism that isnegative for both, and an organism that is positive for one but not the other. Explain the only possible way an organism could bepositive for one but not for the other when using this medium.Directions: Download and Save this document as “BIOL 1020 Unit 2 Module 5 Lab Assessment 3_Lastname”. SAVE YOUR WORK AS A WORD (.doc)DOCUMENT. When you have completed this assessment, submit it to the appropriate Assignments area in Blackboard.Using the VUMIE Lab Manual, the VUMIE website, your notes, and in some cases outside research, answer the following questions. Youranswers must be in the form of complete and grammatically correct sentences. Highlight your responses or use a different font color(todistinguish them from the questions themselves). These questions are ordered as you proceed through the VUMIE lab exercise sections.10 pointsCatalase Test1) Open the Virtual Lab and click the “New” button. Select the “Media Tests 6” unknown from the “Exercise Topic and Subgroup”list. Give the identifier “MT6”. Review the Case Study and record the Gram stain results. Based on shape and grouping, what would youpredict to be the genus of this organism?2) From the “View” list, select “Catalase Video”. Appearing will be a viewer where you can watch the addition of 3% hydrogenperoxide to a smear of fresh growth on a microscope slide. The video can be replayed if needed. Based on the information providedabove, is this organism catalase positive or negative? (Circle or highlight)Positive Negative3) What evidence supports your interpretation?4) What would the video look like if the result was opposite to that portrayed in the video?5) Record the results in the software and watch the Identification Matrix for any changes. Click on View and select Lab Report todetermine how many species of bacteria were unknown based on the result you recorded. How many bacterial species were eliminated?6) Would strict aerobes or strict anaerobes be more likely to possess catalase? Explain your reasoning.